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. 2020 Jul 19;26:e923622-1–e923622-11. doi: 10.12659/MSM.923622

Table 1.

Primer sequences and siRNA Sequences used for quantitative RT-PCR.

Name Sequences (5′-3′)
DANCR Forward: CGTACTAACTTGTAGCAACC
Reverse: TCAGCTGCATTGAGTTAGCG
PTEN Forward: ACTATTCCCAGTCAGAGGCG
Reverse: GAACTTGTCTTCCCGTCGTG
GAPDH Forward: ACTCACTCAAGATTGTCAGCA
Reverse: GGCCATCACGCCACAGCTTT
si-DANCR GCUGGUAUUUCAAUUGACU
si-EZH2 GCAGAAAGAUACAGCUGAA
si-PTEN GACAGAUGUUUGAAGAUAA
si-NC UUCUCCGAACGUGUCACGU

siRNA – small interfering RNA; RT-PCR – real-time polymerase chain reaction; DANCR – differentiation antagonizing non-protein coding RNA; PTEN – phosphatase and tensin homolog; GAPDH – glyceraldehyde 3-phosphate dehydrogenase; si-DANCR – specific siRNA targeting DANCR; si-EZH2 – specific siRNA targeting EZH2; si-PTEN – specific siRNA targeting PTEN; si-NC – scrambled negative control.