Table 1.
Name | Sequences (5′-3′) |
---|---|
DANCR | Forward: CGTACTAACTTGTAGCAACC Reverse: TCAGCTGCATTGAGTTAGCG |
PTEN | Forward: ACTATTCCCAGTCAGAGGCG Reverse: GAACTTGTCTTCCCGTCGTG |
GAPDH | Forward: ACTCACTCAAGATTGTCAGCA Reverse: GGCCATCACGCCACAGCTTT |
si-DANCR | GCUGGUAUUUCAAUUGACU |
si-EZH2 | GCAGAAAGAUACAGCUGAA |
si-PTEN | GACAGAUGUUUGAAGAUAA |
si-NC | UUCUCCGAACGUGUCACGU |
siRNA – small interfering RNA; RT-PCR – real-time polymerase chain reaction; DANCR – differentiation antagonizing non-protein coding RNA; PTEN – phosphatase and tensin homolog; GAPDH – glyceraldehyde 3-phosphate dehydrogenase; si-DANCR – specific siRNA targeting DANCR; si-EZH2 – specific siRNA targeting EZH2; si-PTEN – specific siRNA targeting PTEN; si-NC – scrambled negative control.