Table 3.
Effect of osmolarity on fliC::lacZ gene transcription in E. coli grown in buffered pH 5.5 and 7.0 LB media with different osmolarities
| E. coli strain | NaCl (mM) | β-gal Activitya | |
|---|---|---|---|
| pH 5.5 | pH 7.0 | ||
| MC4100/pNK2–29 | 0 | 308 ± 104b | 1,132 ± 130 |
| MC4100/pNK2–29 | 100 | 338 ± 128 | 806 ± 41 |
| MC4100/pNK2–29 | 200 | 251 ± 68.5 | 689 ± 173 |
| MC4100/pNK2–29 | 400 | 62 ± 22.0 | 454 ± 71 |
| NU149 LacZ1/pNK2–29 | 0 | 442 ± 72 | 1,353 ± 98 |
| NU149 LacZ1/pNK2–29 | 100 | 418 ± 61 | 976 ± 52 |
| NU149 LacZ1/pNK2–29 | 200 | 293 ± 43 | 811 ± 75 |
| NU149 LacZ1/pNK2–29 | 400 | 147 ± 39 | 489 ± 61 |
β-galactosidase activity measured as Miller units.
Data represents the mean ± standard deviation from three separate runs.