Table 4.

Assessing a gadE and mutation and complementation on fliC::lacZ gene transcription in E. coli grown in buffered pH 5.5 and 7.0 LB media with different osmolarities

E. coli strain	β -gal Activitya
	pH 5.5	pH 5.5+b	pH 7.0	pH 7.0+
EK227/pNK2–29	540±51c	165±59	1,520±144	540±66
EF1007 /pNK2–29d	1,742±109	470±106	2,196±173	681±135
EF1083/pNK2–29	295±93	131±20	794±145	404±41

^aβ-galactosidase activity measured as Miller units.

^b+ = 400 mM added NaCl.

^cData represents the mean ± standard deviation from three separate runs.

^dEF1007 is ΔgadE and EF1083 is ΔgadE/pGadE+.