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. Author manuscript; available in PMC: 2020 Jul 29.
Published in final edited form as: Antiviral Res. 2019 Apr 26;167:98–103. doi: 10.1016/j.antiviral.2019.04.011

Fig. 3. Effect of Curcumin on KSHV lytic replication.

Fig. 3.

BCBL-1 cells were treated with a wide range of concentrations of Curcumin 3 h after being induced by TPA for viral lytic replication. (A) Whole cell extracts were prepared 48 h post-induction and subjected to Western blot analysis with anti-RTA and anti-K8 monoclonal antibodies. (B) Total DNA was purified 48-h post-induction and intracellular KSHV DNA replication was determined using real-time quantitative PCR (blue). Five days post-induction, cell culture media were collected and extracellular virions (green) were determined as described in Materials and Methods (green). Uninduced BCBL-1 cells were treated with curcumin for 120 h and cell cytotoxicity (Orange) were assessed by Trypan blue-staining procedure using a Countstar instrument. The mean values from three independent experiments and standard deviations are presented on the y-axis of dose-response curves. IC50, EC50 and CC50 are calculated using GraphPad Prism software.