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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Gastroenterology. 2019 May 30;157(3):744–759.e4. doi: 10.1053/j.gastro.2019.05.057

Figure 4. Intestine-specific knockout of PPARα increased the expression of DNMT1 and PRMT6.

Figure 4.

(A) The mRNA levels of methyltransferases in mouse colon tumors (T) and adjacent non-tumor tissues (NT). N = 5/group. (B) The mRNA levels of methyltransferases in the colon samples from Pparafl/fl and PparaΔIE mice. N=5/group. (C) Western blot analysis of indicated proteins in mouse colon tumors (T) and adjacent non-tumor tissues (NT). (D) Western blot analysis of indicated proteins in colon samples from Pparafl/fl and PparaΔIE mice. (E) The mRNA levels of Ppara, Acox1, Dnmt1, and Prmt6 in colon tumors (T) and adjacent non-tumor tissues (NT) from AOM-administered Pparafl/fl and PparaΔIE mice (n=5/group). (F) The mRNA (n=3) and (G) protein levels of DNMT1 and PRMT6 in primary intestinal epithelial cells isolated from Pparafl/fl and PparaΔIE mice. (H) Primary intestinal epithelial cells isolated from wild-type mice were cultured and infected with control adenovirus (Ad-GFP), adenovirus expressing PPARα (Ad-PPARα), or not infected (No-Ad). The mRNA (n=2 for No-Ad group, and n=4 for Ad-GFP group and Ad-PPARα group) and (I) protein levels of indicated genes were analyzed. * P < 0.05, ** P < 0.01, *** P < 0.001.