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. Author manuscript; available in PMC: 2020 Sep 1.
Published in final edited form as: Gastroenterology. 2019 May 30;157(3):744–759.e4. doi: 10.1053/j.gastro.2019.05.057

Figure 5. DNMT1 and PRMT6 suppressed the expression of p21 and p27 by DNA methylation and histone arginine methylation, respectively.

Figure 5.

(A) The mRNA levels of tumorigenesis related genes in mouse colon tumors (T) and adjacent non-tumor tissues (NT). N = 5/group. (B) The mRNA levels of tumorigenesis related genes in the colon samples from Pparafl/fl and PparaΔIE mice. N=5/group. (C) The mRNA (n=4/group) and (D) protein levels of indicated genes in HCT116 cells transfected with empty vector pcDNA3 or plasmid expressing DNMT1 (pDNMT1), or exposed to DMSO (Ctrl) or 5-Aza. (E) The mRNA (n=4/group) and (F) protein levels of indicated genes in HCT116 cells transfected with empty vector pCMV or plasmid expressing PRMT6 (pPRMT6), or administered with DMSO (Ctrl) or EPZ020411 (EPZ). (G) Genomic DNA was isolated from HCT116 cells described in (D) and subjected to MeDIP analysis (n=3/group). (H) Genomic DNA was isolated from colon tissues of mice described in Figure 3A and subjected to MeDIP analysis (n=3/group). Values were calculated as percentages of input DNA and expressed as relative enrichment compared to control group, which was equated to 1. (I) Histone ChIP assay was performed using chromatins prepared from HCT116 cells described in (F) (n=3/group). (J) Histone ChIP assay was performed using chromatins prepared from colon tissues of mice described in Figure 3A (n=3/group). Values were expressed as relative enrichment compared to histone H3. * P < 0.05, ** P < 0.01, *** P < 0.001.