Table 1.
Sequences of primers and probe for RT-RAA Assay for SARS-CoV-2.
| Primer/Probes | Sequence (5′-3′) | Genome positiona |
|---|---|---|
| SARS-CoV-2-F | CAGTTCAAGAAATTCAACTCCAGGCAGCAGTAG | 28,849-28881 |
| SARS-CoV-2-R SARS-CoV-2-P |
CAGTTTGGCCTTGTTGTTGTTGGCCTTTAC | 29,009-28980 |
| CAGACATTTTGCTCTCAAGCTGGTTCAATC [FAM-dT](THF)[BHQ-dT]CAAGCAGCAGCAAAG (C3-spacer) | 28,979-28932 | |
Note. Probe modifications: FAM: 6-Carboxyfluorescein; THF: tetrahydrofuran; BHQ: black hole quencher; C3 spacer: 3′ phosphate blocker.
a
The genome position refers to the nucleocapsid protein gene (N gene) from BetaCoV/Wuhan/WIV04/2019 (EPI_ISL_402,124).