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. 2020 Jul 2;46(3):989–1002. doi: 10.3892/ijmm.2020.4666

Figure 2.

Figure 2

miR-589-3p mimic reversed the effect of TINCR overexpression on miR-589-3p expression. (A and B) RT-qPCR was used to detect TINCR expression level in transfected MCF-7 and MDA-MB-231 cells. GAPDH was used as an internal reference. (C and D) RT-qPCR was used to detect the expression level of miR-589-3p in transfected MCF-7 and MDA-MB-231 cells. U6 was used as an internal reference. All experiments were repeated three times. **P<0.01 vs. pc-Control; ##P<0.01 vs. miR-589-3p mimic control; ΘΘP<0.001 vs. pc-TINCR; ^^P<0.01 vs. miR-589-3p mimic; ΔΔP<0.01 vs. si-Control; ωP<0.05 and ωωP<0.01 vs. miR-589-3p inhibitor; ρρP<0.01 vs. miR-589-3p inhibitor control; ΓΓP<0.01 vs. si-TINCR. miR, microRNA; RT-qPCR, reverse transcription-quantitative PCR; TINCR, tissue differentiation inducing non-protein coding RNA; si, small interfering.