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. 2020 Jul 2;46(3):989–1002. doi: 10.3892/ijmm.2020.4666

Figure 7.

Figure 7

miR-589-3p regulated the activation of Akt pathway by targeting IGF1R in breast cancer cells. (A and B) Reverse transcription-quantitative PCR was used to detect IGF1R and Akt expression levels. (C-E) Western blotting used to detect the protein levels of IGF1R, p-AKT and Akt in MCF-7 cells. (F-H) Western blotting used to detect the protein levels of IGF1R, p-AKT and Akt in MDA-MB-231 cells. All experiments were repeated three times. GAPDH was used as an internal reference. **P<0.01 vs. NC; ##P<0.01 vs. IGF1R; ΘΘP<0.01 vs. IGF1R + miR-589-3p mimic; ΔΔP<0.01 vs. siNC; ρρP<0.01 vs. siIGF1R; ΓΓP<0.01 vs. siIGF1R + miR-589-3p inhibitor. IGF1R, insulin-like growth factor 1 receptor; miR, microRNA; NC, negative control; p, phospho; si, small interfering.