Figure 4.

Effects of treatments with Doxo alone and in combination with radicicol on catalase (A and B) gene and (C) protein expression in the H9c2 cells. Doxo (1 µM) was administered for 3 and 6 h, and where indicated, radicicol (1 µM) was administered 30 min prior to Doxo. (A) CAT mRNA levels analyzed by RT-qPCR, with the data normalized to the HPRT1 gene, The data are presented as the means ± SD for at least 3 independent experiments, which each performed in triplicate. (B) Representative agarose gel of RT-qPCR products of the data shown in (A). (C) Catalase levels in the cytosol, using flow cytometry for the H9c2 cells. Data are means ± SEM for the percentages of catalase-positive cells, from at least 3 independent experiments, each performed in duplicate. ^*P<0.05, ^**P<0.005 vs. control, °°P<0.005 vs. cells treated with Doxo under the same conditions (one-way ANOVA and multiple comparisons by Bonferroni's test). Doxo, doxorubicin; Rad, radicicol.