Figure 5.

Effects of treatment with Doxo alone and in combination with radicicol on the apoptotic pathway of the H9c2 cells. Doxo (1 µM) was administered for 3 and 6 h, and where indicated, radicicol (1 µM) was administered 30 min prior to Doxo. (A) The H9c2 cells were stained by propidium iodide and fluorescence of individual nuclei was measured by flow cytometry. Data are the means ± SEM for the percentages of hyplodiploid nuclei, from at least 3 independent experiments, each performed in duplicate. (B and C) Caspase 9 (B) and procaspase 3 (C) expression detected by western blot analysis, with Gapdh expression as the loading control. Insets: Representative western blots. Data are the means ± SEM, from at least 3 independent experiments, each performed in duplicate. ^*P<0.05, ^**P<0.005, vs. control; °P<0.05 and °°P<0.005 vs. cells treated with Doxo under the same conditions (one-way ANOVA and multiple comparisons by Bonferroni's test). Doxo, doxorubicin; Rad, radicicol.