Figure 6.

Effects of treatment with Doxo alone and in combination with radicicol on NF-κB activation of the H9c2 cells. Doxo (1 µM) was administered for 3 and 6 h, and where ° indicated, radicicol (1 µM) was administered 30 min prior to Doxo. (A) iKKα expression was examined by western blot analysis, with Gapdh expression as the loading control. Insets: Representative western blots. (B) p-IKB levels using flow cytometry of the H9c2 cells. Data are the means ± SEM for the percentages of pIKB-positive cells, from at least 3 independent experiments, each performed in duplicate. ^*P<0.05, ^**P<0.005 vs. control; °P<0.05 vs. cells treated with Doxo under the same conditions (one-way ANOVA and multiple comparisons by Bonferroni's test). Doxo, doxorubicin; Rad, radicicol.