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. 2020 Jun 19;46(3):965–976. doi: 10.3892/ijmm.2020.4651

Figure 5.

Figure 5

miR-143 downregulation enhanced the osteogenic differentiation of human adipose-derived mesenchymal stem cells through activation of the ERK1/2 signaling pathway. Cells were treated with antagomir-143 or antagomir NC, with or without 10 µmol/l U0126 or PD98059 for 15 min. (A) Protein expression levels of p-ERK1/2 and ERK1/2, detected by western blot analysis and quantified using ImageJ software. (B) Graphical representation of the optical density following Alizarin Red S staining at day 21 of culture. (C) ALP activity assessed by colorimetric assay. (D) Runx2, (E) BSP, (F) OPN and (G) osteo-calcin mRNA levels were assessed by reverse transcription-quantitative polymerase chain reaction analysis. Data represent the mean ± standard deviation of three independent experiments. *P<0.05 and **P<0.01, vs. odM group; ##P<0.01 vs. antagomir-143 + odM group. miR, microRNA; ERK1/2, extracellular-signal regulated kinase 1/2; NC, negative control; ALP, alkaline phosphatase; Runx2, Runt-related transcription factor-2; BSP, bone sialoprotein; OPN, osteopontin; odM, osteogenic differentiation medium.