Figure 7.

miR-143 negatively regulated k-Ras/MEK/ERK signaling pathway during osteogenic differentiation of hADSCs. hADSCs were transfected with agomir-143, antagomir-143 or the corresponding NC, and osteogenic differentiation medium was added. (A) Western blot analysis was used to assess the protein expression levels of k-Ras, p-Raf/1, Raf/1, p-MEK1/2, MEK1/2, p-ERK1/2 and ERK1/2, with β-actin used as an internal control. (B) The bands were semi-quantitatively analyzed using ImageJ software and normalized to β-actin density. Data represent the mean ± standard deviation of three independent experiments. ^**P<0.01 and ^##P<0.01. miR, microRNA; hADSCs, human adipose-derived mesenchymal stem cells; MEK, mitogen-activated protein kinase; ERK, extracellular-signal regulated kinase; NC, negative control.