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. 2019 Oct 25;5:100033. doi: 10.1016/j.tcsw.2019.100033

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Fig. 2 — Liquid chromatography-electrospray ionization-mass spectrometry of a Ba(OH)₂ hydrolysate of FM966 fibres sampled 20 DPA. Diagnostic ion traces of Hyp-Araf_2-4 (extracted ion traces of [M + H⁺|Na⁺]⁺ 396/418 (Hyp-Araf₂), 528/550 (Hyp-Araf₃) and 660/682 (Hyp-Araf₄ each eluting as twin peaks due to the C-4 R/S stereochemistry of hydroxyproline (Hyp)), were monitored by liquid chromatography-electrospray ionization-mass spectrometry of a Ba(OH)₂ hydrolysate of FM966 fibres sampled 20 DPA. Three technical replicates were analysed. The table insert shows a monosaccharide profile of a fraction enriched in Hyp-arabinosides and arabinose isolated by size exclusion chromatography using a BioRad P2 column.