Table 2.
Fitted parameters of the energy landscape for the interaction of functionalized AFM dendritips with specific probe to cell wall polysaccharides. The Friddle-Noy-de Yoreo model (Friddle et al., 2012) has been used to determine the energy landscape of the intermolecular interaction between each couple of polysaccharide with its specific probes covalently attached on a AFM dendritip. Details about the nature of each of the parameters is described in the Results section.
| Functionalized AFM dendritip | Polysaccharides coated on epoxide-glass slide | Xt (Å) | feq (pN) | koff (s−1) | τ0 (ms) | ΔG (kcal.mol−1) |
|---|---|---|---|---|---|---|
| Anti-β-1,3-glucan antibodies | β-1,3-Laminarin | 0.6 ± 0.2 | 36.2 ± 1.0 | 732.4 ± 10.4 | 1.37 | −5.3 |
| Anti-β-1,6-glucan antibodies | β-1,6-Gentiobiose | 0.3 ± 0.1 | 52.7 ± 4.1 | 1046.7 ± 197.6 | 0.96 | −11.0 |
| Concanavalin A | α-Mannan | 0.2 ± 0.1 | 35.3 ± 1.0 | 569.9 ± 15 | 1.75 | −4.2 |
| Wheat germ agglutinin | β-1,4-Penta-N-acetylchitopentaose | 0.1 ± 0.04 | 88.1 ± 2.9 | 113.6 ± 10.1 | 8.81 | −55.1 |