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. 2020 Jul 28;16:99. doi: 10.1186/s13007-020-00641-1

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Substrate specificity assay of AtGALS1. Dose–response curve for the binding interaction between YFP-AtGALS1 and a UDP-Ara_f, or b UDP. Concentration of YFP-AtGALS1 was kept constant, while the ligand varied from 2.5 mM to 76.3 nM. Data were fitted to K_d model and confidence interval of the K_d is calculated from the variance of the fitted parameter, derived from Levenberg–Marquardt fit-algorithm. Error Bar: SD, n = 3. Values are the average ± 68% confidence