Table 1.

Fluorescence counts and aggregation status of YFP-AtGALS1 microsomes in different incubation buffers

Buffer composition	Fluorescence counts	Aggregation observed
HEPES pH 7.0, 50 mM NaCl, 5 mM MnCl2, 1% Tx100	1247	(+)
PBS pH 7.4, 5 mM MnCl2, 0.05% Tween	479	−
MES pH 6.5, 5 mM MnCl2, 125 mM KCl, 1% Tx100	974	+
MES pH 6.5, 5 mM MnCl2, 1% Tx100	1263	−
MES pH 6.5, 5 mM MnCl2, 0.05% Tween	101	++
MES pH 6.5, 5 mM MnCl2, 0.5% Tween	174	+
MES pH 6.5, 5 mM MnCl2, 2% Tween	272	−

MES buffer with 1% Triton X-100 provides highest fluorescence counts and did not produce bumpy thermograph curve as indication of protein aggregation. Tween as detergent with concentration from 0.05 to 2% did not work well with MES. Thermographs corresponding to the different conditions are shown in Additional file 1: Figure S1