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. 2020 Apr 21;6:100040. doi: 10.1016/j.tcsw.2020.100040

Table 1.

Primers used for the generation of over-expression constructs. The restriction site is identified in bold type.

Primer Plasmid Sequence (5′-3′)
Rv0183 sense pVV16 GATCGACTCATATGACTACCACCCGGACTGAACG
pET28a
Rv0183 Anti-sense pVV16 GATCGATCGGATCCCCAACCGCTCGGTGAGCCAG
pET28 GATCGATCGGATCCCTACAACCGCTCGGTGAGC