Table 1.
Primers used for the generation of over-expression constructs. The restriction site is identified in bold type.
| Primer | Plasmid | Sequence (5′-3′) |
|---|---|---|
| Rv0183 sense | pVV16 | GATCGACTCATATGACTACCACCCGGACTGAACG |
| pET28a | ||
| Rv0183 Anti-sense | pVV16 | GATCGATCGGATCCCCAACCGCTCGGTGAGCCAG |
| pET28 | GATCGATCGGATCCCTACAACCGCTCGGTGAGC |