Table 1.
PCR conditions and primer sequences
| Primer | Sequence 5′ - 3’ |
Amplicon size (bp) | Cycling conditions |
|---|---|---|---|
| 16SrDNA_F | CCTACGGGNGGCWGCAG | 465 | 1 × 95 °C, 3’ |
| 16SrDNA_R | GACTACHVGGGTATCTAATCC | 35 × 95 °C, 15″; 55 °C, 20″; 72 °C, 30″ | |
| ermB_F | AAGGGCATTTAACGACGAAACTG | 438 | 1 × 95 °C 3’ |
| ermB_R | ATTTATCTGGAACATCTGTGGTATG | 40 × 95 °C 15″, 60 °C 20″, 72 °C 30″ | |
| ermF_F | CGACACAGCTTTGGTTGAAC | 120 | 1 × 95 °C 3′ |
| ermF_R | TTTGACACCACTTTGAAAGGAAA | 40 × 95 °C 15″, 58 °C 20″, 72 °C 30″ | |
| mefA | CCTGCAAATGGCGATTATTT | 199 | 1 × 95 °C 3′ |
| mefA | AATAGCAAGCACTGCACCAG | 40 × 95 °C 15″, 58 °C 20″, 72 °C 30″ |