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. 2020 Jul 28;19:151. doi: 10.1186/s12934-020-01409-x

Fig. 1.

Fig. 1

Detection of α-amylase activity from recombinant strains of B. methanolicus incubated at 50 °C. In the plate assay, hydrolysis of starch by α-amylase is seen as clearing zones around the colonies, visualized by addition of Lugol’s solution after 24 h incubation. Recombinant strains tested are: Plate a: EV (emtpy vector), used as control (A), spBl-amyBl (B), spBs-amyBs (C), spBm-amyBm (D) and spGs-amyGs (E). Plate b: Control strain EV (F), spGs-amyGs (G), spGs-amyBl (H) and spGs-amyBs (I)