Table 1.
Proteins with native signal peptides used in this study
| Product | Native organism | Protein | Signal peptide | |||
|---|---|---|---|---|---|---|
| Accession number | Temperature optimuma (°C) | SignalP-5.0 (probability) | PrediSI (score) | Cleavage sitec (amino acid) | ||
| α-Amylase | Sec/SPI | |||||
| amyL | B. licheniformis | BL00499 | 90 | 0.9792 | 0.9872 | 28/29, 28 |
| amyE | B. subtilis | BSU03040 | 50 | 0.9718 | 0.6027 | 29, 33/34 |
| amyS | G. stearothermophilus | GT50_07800 | 65 | 0.9807 | 1.0000 | 34/35, 34 |
| amy | B. methanolicus | BMMGA3_04345 | ND | 0.9931 | 0.6850 | 23/24, 24 |
| Protease | ||||||
| apr | B. licheniformis | BLI_RS05490 | 50 | 0.9935 | 0.9128 | 29/30, 29 |
| aprE | B. subtilis | GP2222_11200 | 60b | 0.9929 | 0.8976 | 29/30, 29 |
| apr | G. stearothermophilus | GT50_00190 | NC | 0.6871 | 0.6283 | 30/31, 30 |
Signal peptides were analyzed by SignalP-5.0 and PrediSI [69, 71]
ND not determined, NC not characterized. To the best of the authors’ knowledge
a Temperature optimum of proteins previously determined. See text for details and references
b Temperature optimum for homolog protease-gene in B. subtilis A26
c As predicted by SignalP-5.0 and PrediSI, respectively [69, 71]