1.
引物序列和产物长度
| 基因 | 序列 | 产物长度 |
| β-actin | 5'-AGGCTGTGTTGTCCCTGTATG-3' | 203 bp |
| 5'-AGCTGTGGTGGTGAAGCTGTA-3' | ||
| Ampk | 5'-GGCGTGTGAAGATCGGACA-3' | 235 bp |
| 5'-GTTGGAGTGCTGATCACTTGG-3' | ||
| Adipor1 | 5'-GGTGGTCTTCGGGATGTTCTT-3' | 213 bp |
| 5'-ATGGAGAGGTAGATGAGCCGT-3' | ||
| Adipor2 | 5'-TACACACAGAGACGGGCAAC-3' | 194 bp |
| 5'-GCAGTACACCGTGTGGAAGA-3' | ||
| Irs1 | 5'-GTGGCCAGCCAGGCTATTTA-3' | 111 bp |
| 5'-ACTCTTCCGAGCCAGTCTCT-3' | ||
| Irs2 | 5'-GCCACCGTGGTGAAAGAGTA-3' | 103 bp |
| 5'-AGCGTTGGTTGGAAACATGC-3' | ||
| Nr1h3 | 5'-CCAGGATGCCCCAATCAACT-3' | 236 bp |
| 5'-GAAGAATCCCTTGCAGCCCT-3' | ||
| Ppargc1a | 5'-GACATGTGCAGCCAAGACTC-3' | 213 bp |
| 5'-TATGTTCGCGGGCTCATTGT-3' | ||
| Ppara | 5'-GTCCCTCGGAGAGGAGAGTT-3' | 170 bp |
| 5'-TGCCAGGGGACTCATCTGTA-3' | ||
| Pparg | 5'-CTGCGTCCCCGCCTTATTAT-3' | 293 bp |
| 5'-GGCATTGTGAGACATCCCCA-3' | ||
| Srebf1 | 5'-CGCTCTTGACCGACATCGAA-3' | 110 bp |
| 5'-GCCTGTGTCTCCTGTCTCAC-3' | ||
| Cpt1a | 5'-CCCTGTGAATACTTGGGCGT-3' | 275 bp |
| 5'-ATGGGCCCCTTCTTTAAGGC-3' |