Fig. 10.

qRT-PCR validation of expression levels of the overlapping DEGs from MAPK signaling pathway a, TGF-β signaling pathway b, TNF signaling pathway c, and FoxO signaling pathway d in the C50336-VS-C50336::dam group. J774A.1 cells were pre-treated with LPS (1 μg/mL, 5 h) and then infected with C50336 or C50336::dam (cultured with IPTG) at an MOI of 20 for 4 h. Each group comprised three independent replicates. The cells were then collected, and the total RNA was isolated for qRT-PCR. The mRNA expression level was normalized against the mouse GAPDH transcript. Data are presented as mean ± SEM of log₂ (fold change) of triplicate samples