Fig. 11.

The deletion of Dam blocked the Jnk pathway in infected J774A.1 cells. J774A.1 cells were pre-treated with LPS (1 μg/mL, 5 h) and then infected with C50336, C50336Δdam, C50336Δdam::dam, C50336Δdam-pMMB207, C50336::dam, or C50336-pMMB207 at an MOI of 20 for 4 h, uninfected cells was used as a negative control (Blank). Bacteria bearing pMMB207 plasmids were cultured without IPTG. The activation of caspase-1, phosphorylated Jnk (P-Jnk), phosphorylated p38 (P-p38), and phosphorylated ERK1/2 (P-ERK1/2) were analyzed by immunoblotting. β-actin was blotted as a loading control. Molecular mass markers in kDa are indicated on the right. Original images of immunoblotting were shown in Fig. S7