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. 2020 Jul 28;20:226. doi: 10.1186/s12866-020-01919-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 7 — Differentially expressed genes (DEGs) between J774A.1 cells infected with SE. J774A.1 cells were pre-treated with LPS (1 μg/mL, 5 h) and then infected with C50336, C50336Δdam, or C50336::dam (cultured with IPTG) at an MOI of 20 for 4 h. Each group comprised three independent replicates. The cells were then collected, and the total RNA was isolated for RNA sequencing. Volcano plot of all DEGs between the C50336-infected group and the C50336Δdam-infected group a, the C50336-infected group and the C50336::dam-infected group b, and the C50336Δdam-infected group and the C50336::dam-infected group c. Genes with log2 (fold change) ≤ − 1 and p-value < 0.05 are marked with blue dots, and genes with log2 (fold change) ≥ 1 and p-value < 0.05 are marked with red dots. d. Venn diagram displaying the number of overlapping DEGs in J774A.1 cells infected with different SE