Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Reference |
|---|---|---|
| Escherichia coli | ||
| X7213 λpir | Host for π requiring plasmids, conjugal donor | Labortaory collection |
| X7213 λpir-pSC189 | X7213 λpir with pSC189, Kmr, Cmr | This study |
| SalmonellaEnteritidis | ||
| C50336 | Wild type | Obtained from Chinese National Institute for the Control of Pharmaceutical and Biological |
| C50336∆dam | C50336, In-frame deletion in dam | This study |
| C50336∆invC | C50336, In-frame deletion in invC | This study |
| C50336∆hilD | C50336, In-frame deletion in hilD | This study |
| C50336∆prgH | C50336, In-frame deletion in prgH | This study |
| C50336∆spaN | C50336, In-frame deletion in spaN | This study |
| C50336∆dam::dam | C50336∆dam with pMMB207 expressing the dam gene,Cmr | This study |
| C50336∆ invC::invC | C50336∆ invC with pMMB207 expressing the invC gene,Cmr | This study |
| C50336∆hilD::hilD | C50336∆ invC with pMMB207 expressing the hilD gene,Cmr | This study |
| C50336∆ prgH::prgH | C50336∆ prgH with pMMB207 expressing the prgH gene,Cmr | This study |
| C50336∆ spaN::spaN | C50336∆dam with pMMB207 expressing the dam gene,Cmr | This study |
| C50336-pMMB207 | C50336 with pMMB207,Cmr | This study |
| C50336::dam | C50336 with pMMB207 expressing the dam gene,Cmr | This study |
| C50336::prgH | C50336 with pMMB207 expressing the prgH gene,Cmr | This study |
| C50336∆dam-pMMB207 | C50336∆dam with pMMB207,Cmr | This study |
| C50336∆prgH-pMMB207 | C50336∆prgH with pMMB207,Cmr | This study |
| C50336-pCX340 | C50336 with pCX340, Tetr | This study |
| C50336-pCX340-dam | C50336 with pCX340 expressing the dam gene, Tetr | This study |
| C50336-pCX340-invC | C50336 with pCX340 expressing the invC gene, Tetr | This study |
| C50336-pCX340-prgH | C50336 with pCX340 expressing the prgH gene, Tetr | This study |
| C50336-pCX340-spaN | C50336 with pCX340 expressing the dam gene, Tetr | This study |
| Plasmids | ||
| pSC189 | Transposon delivery vector, R6K, Kmr, Cmr | [74] |
| pDM4 | Suicide vector, pir dependent, R6K, SacBR, Cmr | [75] |
| pMMB207 | IncQ lacIq Δbla Ptac-laclacZa, Cmr | [24] |
| pMMB207-dam | pMMB207 derivative containing dam, Cmr | This study |
| pMMB207-invC | pMMB207 derivative containing invC, Cmr | This study |
| pMMB207-hilD | pMMB207 derivative containing hilD, Cmr | This study |
| pMMB207-prgH | pMMB207 derivative containing prgH, Cmr | This study |
| pMMB207-spaN | pMMB207 derivative containing spaN, Cmr | This study |
| pCX340 | pBR322 derivative, cloning vector used to fuse effectors to TEM-1-β-lactamase, Tetr | [76] |
| pCX340-dam | pCX340 derivative containing dam | This study |
| pCX340-invC | pCX340 derivative containing invC | This study |
| pCX340-prgH | pCX340 derivative containing prgH | This study |
| pCX340-spaN | pCX340 derivative containing spaN | This study |
The antibiotics as follows: kanamycin (Kmr), 100 μg/mL; chloramphenicol (Cmr), 25 μg/mL; tetracycline (Tetr), 12.5 μg/mL