Table 2.
Primers used in this study
| Primer name | Primer sequence (5′ to 3′) | Target |
|---|---|---|
| AB1 | GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC | For transposon insertion sequencing |
| AB2 | GGCCACGCGTCGACTAGTACNNNNNNNNNNCCTGG | For transposon insertion sequencing |
| AB3 | GGCCACGCGTCGACTAGTACNNNNNNNNNNCCTCG | For transposon insertion sequencing |
| ABS | GGCCACGCGTCGACTAGTAC | For transposon insertion sequencing |
| SP1 | GCTGACCGCTTCCTCGTGCTTTACG | For transposon insertion sequencing |
| SP2 | CATCGCCTTCTATCGCCTTCTTGAC | For transposon insertion sequencing |
| pSC189-seq | CGCGAAGTTCCTATTCCGAAGTTCC | For transposon insertion sequencing |
| pDM4-F | GGTGCTCCAGTGGCTTCTGTTTCTA | For deletion mutants |
| pDM4-R | CAGCAACTTAAATAGCCTCTAAT | For deletion mutants |
| dam-up-F | GAGCGGATAACAATTTGTGGAATCCCGGGAGACGCCGAAAGCGACCACCACGACG | For dam deletion mutant |
| dam-up-R | TTGAGAATTACATGCTGACTAACTAATTACACCTT | For dam deletion mutant |
| dam-down-F | AGTCAGCATGTAATTCTCAAGGAGAAGCGGATGAA | For dam deletion mutant |
| dam-down-R | AGCGGAGTGTATATCAAGCTTATCGATACCCGATCTCGCCGATATTGTTCACCTT | For dam deletion mutant |
| dam-in-F | CGAGTGCCTTGTCGAACCTTTTGTG | For dam deletion mutant |
| dam-in-R | ACAGAGCCAGCAGTTCGTCCACCTT | For dam deletion mutant |
| dam-out-F | AACCACGACTGCGGAACCGAAGAAA | For dam deletion mutant |
| dam-out-R | TCGGGTTTATCGAAAATTGCCGACC | For dam deletion mutant |
| invC-up-F | GAGCGGATAACAATTTGTGGAATCCCGGGAGTCCTCCTTACGTCTGTCGATGTCC | For invC deletion mutant |
| invC-up-R | GCGAATGCATTCATCTCATTAGCGACCGACTAAAA | For invC deletion mutant |
| invC-down-F | AATGAGATGAATGCATTCGCTGACCAGAATTAAAG | For invC deletion mutant |
| invC-down-R | AGCGGAGTGTATATCAAGCTTATCGATACCAATGCTTCTGATAAACCGCCAACCT | For invC deletion mutant |
| invC-in-F | GGGAACGCACCGTGTTGAGCCTTAT | For invC deletion mutant |
| invC-in-R | CCAGGACGATATTCTCCCAAGTCAA | For invC deletion mutant |
| invC-out-F | CAGTACCTTCCTCAGCCTTGACCCG | For invC deletion mutant |
| invC-out-R | GCATTACGAAAGCATCGCCATAGTC | For invC deletion mutant |
| hilD-up-F | GAGCGGATAACAATTTGTGGAATCCCGGGAGCTCATGGAGTATAATTTCGGTCGT | For hilD deletion mutant |
| hilD-up-R | AAAAATGTTACATATTATCCCTTTGTTGATGTTAT | For hilD deletion mutant |
| hilD-down-F | GGATAATATGTAACATTTTTTGTATCTGTCACTTA | For hilD deletion mutant |
| hilD-down-R | AGCGGAGTGTATATCAAGCTTATCGATACCTGAAAGACTGTTTTTAATGGTGCGC | For hilD deletion mutant |
| hilD-in-F | GTCAGACTCAGCAGGTTACCATCAA | For hilD deletion mutant |
| hilD-in-R | CATTATGGTTGCCTATGCGTAAAAG | For hilD deletion mutant |
| hilD-out-F | TTCACCGACCTGTATTGGCGTATTT | For hilD deletion mutant |
| hilD-out-R | TTTTGGGGTGTAAATGCTGCTTATT | For hilD deletion mutant |
| prgH-up-F | GAGCGGATAACAATTTGTGGAATCCCGGGACACCAACATCCCAGGTTCGTCACAG | For prgH deletion mutant |
| prgH-up-R | CGTTAAATTACATATATACTGTTAGCGATGTCTGT | For prgH deletion mutant |
| prgH-down-F | AGTATATATGTAATTTAACGTAAATAAGGAAGTCA | For prgH deletion mutant |
| prgH-down-R | AGCGGAGTGTATATCAAGCTTATCGATACCTTCAACAGCCCCGACTCCTTTACGA | For prgH deletion mutant |
| prgH-in-F | GTTTGCTGCTCGTTTGGGATAAGTG | For prgH deletion mutant |
| prgH-in-R | GGCAAGGGTCATTACCAGCAGAAAG | For prgH deletion mutant |
| prgH-out-F | GAACGGCTGTGAGTTTCCATTGCTG | For prgH deletion mutant |
| prgH-out-R | GACGGGCTCTGAGTATTTCTACATC | For prgH deletion mutant |
| spaN-up-F | GAGCGGATAACAATTTGTGGAATCCCGGGAATTGACTTGGGAGAATATCGTCCTG | For spaN deletion mutant |
| spaN-up-R | AATGACATCACATTAAATTATCTCCTCTGACTCGG | For spaN deletion mutant |
| spaN-down-F | TAATTTAATGTGATGTCATTGCGTGTGAGACAGAT | For spaN deletion mutant |
| spaN-down-R | AGCGGAGTGTATATCAAGCTTATCGATACCCGCACGGGAAGTACGAATCAGGAGT | For spaN deletion mutant |
| spaN-in-F | CGACTATGGCGATGCTTTCGTAATG | For spaN deletion mutant |
| spaN-in-R | CAAACGATGTTCAACCTGCGTATTT | For spaN deletion mutant |
| spaN-out-F | AAAGCGTAAGCCGCGTTTTTGGACA | For spaN deletion mutant |
| spaN-out-R | CAATTGATTCAAGCCAGGCAGAGTT | For spaN deletion mutant |
| pMMB207-F | CTCCCGTTCTGGATAATGTT | For complemented mutants |
| pMMB207-R | GGCGTTTCACTTCTGAGTTCG | For complemented mutants |
| pMMB207-dam-F | AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGAAAAAAAATCGCGCTTTTTTGA | For complemented mutant of ∆dam |
| pMMB207-dam-R | TCTCATCCGCCAAAACAGCCAAGCTTTATTTTCTTGCAGGCGTTGCGACT | For complemented mutant of ∆dam |
| pMMB207-invC-F | AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGAAAACACCTCGTTTACTGCAAT | For complemented mutant of ∆ invC |
| pMMB207-invC-R | TCTCATCCGCCAAAACAGCCAAGCTTTAATTCTGGTCAGCGAATGCATTC | For complemented mutant of ∆ invC |
| pMMB207-prgH-F | AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGGAAACATCAAAAGAGAAGACGA | For complemented mutant of ∆prgH |
| pMMB207-prgH-R | TCTCATCCGCCAAAACAGCCAAGCTTTAAAGTGGGCTTGGGAAATACCAA | For complemented mutant of ∆prgH |
| pMMB207-spaN-F | AGCTCGGTACCCGGGGATCCTCTAGCTAAAGGAAGACGTTATGGGCGATGTGTCAGCTGTCAGTT | For complemented mutant of ∆spaN |
| pMMB207-spaN-R | TCTCATCCGCCAAAACAGCCAAGCTTCAGGCGTCATCCTCCTCGCCAGAT | For complemented mutant of ∆spaN |
| pCX340-F | AGACAATCTGTGTGGGCACTCGACC | For β-lactamase TEM-1 fusion plasmid |
| pCX340-R | TTCTGAGAATAGTGTATGCGGCGAC | For β-lactamase TEM-1 fusion plasmid |
| pCX340-dam-F | AAGGAGGAATAACATATGATGAAAAAAAATCGCGCTTTTTTGA | For β-lactamase TEM-1 fusion plasmid |
| pCX340-dam-R | GTGCGAATTCTCCGCGGAGGTACCTTTTCTTGCAGGCGTTGCGACT | For β-lactamase TEM-1 fusion plasmid |
| pCX340-invC-F | AAGGAGGAATAACATATGATGAAAACACCTCGTTTACTGCAAT | For β-lactamase TEM-1 fusion plasmid |
| pCX340-invC-R | GTGCGAATTCTCCGCGGAGGTACCATTCTGGTCAGCGAATGCATTC | For β-lactamase TEM-1 fusion plasmid |
| pCX340-prgH-F | AAGGAGGAATAACATATGATGGAAACATCAAAAGAGAAGACGA | For β-lactamase TEM-1 fusion plasmid |
| pCX340-prgH-R | GTGCGAATTCTCCGCGGAGGTACCAAGTGGGCTTGGGAAATACCAA | For β-lactamase TEM-1 fusion plasmid |
| pCX340-spaN-F | AAGGAGGAATAACATATGATGGGCGATGTGTCAGCTGTCAGTT | For β-lactamase TEM-1 fusion plasmid |
| pCX340-spaN-R | GTGCGAATTCTCCGCGGAGGTACCGGCGTCATCCTCCTCGCCAGAT | For β-lactamase TEM-1 fusion plasmid |