Table 3.
Primers used in qRT-PCR
| Primer name | Primer sequence (5′ to 3′) | Target |
|---|---|---|
| c-Fos-F | CTCCCGTGGTCACCTGTACT | Detection of mRNA |
| c-Fos-R | TTGCCTTCTCTGACTGCTCA | Detection of mRNA |
| Braf-F | GTAGCGCCTGTTCAGTCCTC | Detection of mRNA |
| Braf-R | GAGCAGCCTGAGTGGTTAGG | Detection of mRNA |
| Rasa1-F | CGGGGTCCTTTGTACTTTCA | Detection of mRNA |
| Rasa1-R | TGGTGGTGCAACTGGATAGA | Detection of mRNA |
| Sos2-F | CAGTCCTCTTGCCACACTCA | Detection of mRNA |
| Sos2-R | GTGGAATAGCAGGAGGGTCA | Detection of mRNA |
| Nr4a1-F | CTTGAGTTCGGCAAGCCTAC | Detection of mRNA |
| Nr4a1-R | CGAGGATGAGGAAGAAGACG | Detection of mRNA |
| Elk4-F | AGCTTTGCCAGAAAAGGACA | Detection of mRNA |
| Elk4-R | TGGTGTAAGAGACGCTGTCG | Detection of mRNA |
| Mef2c-F | ACGCCTGTCACCTAACATCC | Detection of mRNA |
| Mef2c-R | AGCTCTCAAACGCCACACTT | Detection of mRNA |
| Cul1-F | GAATTGGGGCTGAATGAAGA | Detection of mRNA |
| Cul1-R | AACTCTCCGCTGTTCCTCAA | Detection of mRNA |
| Tgfbr1-F | GGCGAAGGCATTACAGTGTT | Detection of mRNA |
| Tgfbr1-R | TGCACATACAAATGGCCTGT | Detection of mRNA |
| Rps6kb1-F | GCTGTGGATTGGTGGAGTTT | Detection of mRNA |
| Rps6kb1-R | GCTTGGACTTCTCCAGCATC | Detection of mRNA |
| Sp1-F | TGCAGCAGAATTGAGTCACC | Detection of mRNA |
| Sp1-R | CACAACATACTGCCCACCAG | Detection of mRNA |
| Rbl1-F | AAACCTTGCACCACAAGTACG | Detection of mRNA |
| Rbl1-R | GCACAGGAGACATTTGATCATT | Detection of mRNA |
| Id1-F | CCAGTGGGTAGAGGGTTTGA | Detection of mRNA |
| Id1-R | AGAAATCCGAGAAGCACGAA | Detection of mRNA |
| Id3-F | ACTCAGCTTAGCCAGGTGGA | Detection of mRNA |
| Id3-R | GTCAGTGGCAAAAGCTCCTC | Detection of mRNA |
| Xiap-F | TTGGAACATGGACATCCTCA | Detection of mRNA |
| Xiap-R | TGCCCCTTCTCATCCAATAG | Detection of mRNA |
| Bcl3-F | TTACTCTACCCCGACGATGG | Detection of mRNA |
| Bcl3-R | CCAAGCTTGAAAAGGCTGAG | Detection of mRNA |
| Chuk-F | TGGAGCCTACGAAGCTGTTT | Detection of mRNA |
| Chuk-R | CCCTCATTAGTTGCGGTGTT | Detection of mRNA |
| Dnm1l-F | ACCCGGAGACCTCTCATTCT | Detection of mRNA |
| Dnm1l-R | GGCGAGAAAACCTTGAGATG | Detection of mRNA |
| Akt3-F | GAAACTGGCCACTTCTGCTC | Detection of mRNA |
| Akt3-R | ACTGAGGTGTGGTGGAGACC | Detection of mRNA |
| Itch-F | CATGTGGTTTTGGCAGTTTG | Detection of mRNA |
| Itch-R | TTGTAAGGTGGGAGGTCCAG | Detection of mRNA |
| Pik3ca-F | ACTGTTCAGAGAGGCCAGGA | Detection of mRNA |
| Pik3ca-R | CGGTTGCCTACTGGTTCAAT | Detection of mRNA |
| Pik3r1-F | GCGTGACATGTAGGCTCTCA | Detection of mRNA |
| Pik3r1-R | CAGTTTCCTTGGCTTTGCTC | Detection of mRNA |
| Pten-F | CATAACGATGGCTGTGGTTG | Detection of mRNA |
| Pten-R | CGGGGTAAGGCTGTTTTACA | Detection of mRNA |
| Irs2-F | GTAGTTCAGGTCGCCTCTGC | Detection of mRNA |
| Irs2-R | CAGCTATTGGGACCACCACT | Detection of mRNA |
| Sirt1-F | AGTTCCAGCCGTCTCTGTGT | Detection of mRNA |
| Sirt1-R | CTCCACGAACAGCTTCACAA | Detection of mRNA |
| Klf2-F | GCCTGTGGGTTCGCTATAAA | Detection of mRNA |
| Klf2-R | AAGGAATGGTCAGCCACATC | Detection of mRNA |
| Sgk3-F | ATCCAGATGTCCGAGCATTC | Detection of mRNA |
| Sgk3-R | GAAGAACCTTGCCAAAGCTG | Detection of mRNA |
| Pdpk1-F | GGTCCAGTGGATAAGCGAAA | Detection of mRNA |
| Pdpk1-R | TTTCTGCACCACTTGTGAGC | Detection of mRNA |
| Mdm2-F | TGCAAGCACCTCACAGATTC | Detection of mRNA |
| Mdm2-R | ACACAATGTGCTGCTGCTTC | Detection of mRNA |