Fig. 5.

Smoking-induced changes in the expression of specific human small airway genes related to lung diseases. a Dot plot of expression of lung disease-related genes in the cell populations recovered by brushing small airway epithelium from healthy nonsmokers vs smokers. The categories of the lung diseases are shown on the x-axis and the identities of the cell populations on the y-axis. The size of the dots represents the fraction of the expressing cells in each cell population, and the color intensity represents the average expression level. b-f Box plots of the examples of lung disease-related genes in the major human SAE cell populations. The data are derived from the single-cell RNA-sequencing dataset. The cell types are labeled on the top of each panel. The box plots were constructed using imputed data. b basal cells; c intermediate cells; d club cells; and e ciliated cells. Gene symbols are shown in the Fig. NS – nonsmokers, S – smokers. * p < 0.05, ** p < 0.01, *** p < 0.001 with Bonferroni correction