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. Author manuscript; available in PMC: 2020 Jul 29.
Published in final edited form as: Cell Rep. 2020 Jun 23;31(12):107810. doi: 10.1016/j.celrep.2020.107810

Figure 4. HIV-1 Infection Induces mTOR-Dependent Acetyl-Lys (40) α-Tubulin Formation in Activated CD4 T Cells.

Figure 4.

(A and B) Resting CD4 T cells or CD4 T cells stimulated with α-CD3/CD28 for 3 days in the absence or presence of mTORi pretreatment were either mock infected (A) or infected with HIV-1-VSV (B) for 4 h. Then, cells were fixed and stained with α-pan-tubulin and α-acetyl-Lys (40) α-tubulin antibodies.

(C) Quantitation of fluorescence signal intensity from images in (A) and (B). Data derived from independent experiments using CD4 T cells from 3 different donors. Means and SDs of data are shown. The significance of differences was determined by 2-tailed Student’s t tests. *p < 0.05, **p < 0.01, and ***p < 0.005.