KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| CAD | Cell Signaling Technology | Cat# 11933 |
| p-CAD | Cell Signaling Technology | Cat# 12662 |
| RRM1 | Cell Signaling Technology | Cat# 8637 |
| thymidylate synthase | Cell Signaling Technology | Cat# 9045 |
| 4E-BP1 | Cell Signaling Technology | Cat# 9452 |
| P-4E-BP1 | Cell Signaling Technology | Cat# 2855 |
| S6 | Cell Signaling Technology | Cat# 2217 |
| p-S6 | Cell Signaling Technology | Cat# 5364 |
| NDRG1 | Cell Signaling Technology | Cat# 5196 |
| P-NDRG1 | Cell Signaling Technology | Cat# 5482 |
| P-S6K1 | Cell Signaling Technology | Cat# 9234 |
| AKT | Cell Signaling Technology | Cat# 9272 |
| p-AKT | Cell Signaling Technology | Cat# 4060 |
| c-Myc | Cell Signaling Technology | Cat# 5605 |
| ASCT2 | Cell Signaling Technology | Cat# 8057 |
| LDHA | Cell Signaling Technology | Cat# 3582 |
| acetyl-α-tubulin | Cell Signaling Technology | Cat# 5335 |
| α-tubulin | Cell Signaling Technology | Cat# 2125 |
| ACLY | Cell Signaling Technology | Cat# 4332 |
| p-ACLY | Cell Signaling Technology | Cat# 4331 |
| IMPDH1 | Novus | Cat# NBP-52933 |
| GLUT1 | Millipore | Cat# 07-1401 |
| SREBP-1 | Santa Cruz | Cat# sc-367 |
| citrate synthase | Santa Cruz | Cat# sc-390693 |
| PGD | Proteintech | Cat# 14718-1-AP |
| RPE | Proteintech | Cat# 12168-2-AP |
| MTHFD2 | Proteintech | Cat# 12270-1-AP |
| GAPDH | Sigma | Cat# G9545 |
| CD3-BV510 | BD Biosciences | Cat# 563109 |
| CD71-AF700 | BD Biosciences | Cat# 563769 |
| Bcl-2-PE | BD Biosciences | Cat# 340651 |
| pStat5-PE-CF594 (pY694) | BD Biosciences | Cat# 562501 |
| pS6-AF488 | Cell Signaling Technology | Cat# 5018 |
| Ki-67-BV421 | Biolegend | Cat# 350505 |
| CD4-PE-Cy7 | Biolegend | Cat# 344612 |
| Anti-Tubulin antibody | Abcam | Cat# ab6161 |
| Biological Samples | ||
| Leukopaks (Buffy Coats) | Lifesouce, Rosemont, IL | N/A |
| Chemicals, Peptides, and Recombinant Proteins | ||
| VU0359595 | Cayman Chemical | Cat# 10955 |
| Rapamycin | Cayman Chemical | Cat# 13346 |
| TAK-228/INK128 | Cayman Chemical | Cat# 11811 |
| Mycophenolic Acid | Cayman Chemical | Cat# 21716 |
| AZD8055 | Selleckchem | Cat# 16978 |
| AZD2014 | Selleckchem | Cat#.S2783 |
| 2′-Deoxyadenosine monohydrate | Sigma | Cat# D8668 |
| 2′-Deoxycytidine hydrochloride | Sigma | Cat# D0776 |
| 2′-Deoxyguanosine monohydrate | Sigma | Cat# D0901 |
| Thymidine | Sigma | Cat# T1895 |
| Guanosine | Sigma | Cat# G6264 |
| 6-Diazo-5-oxo-L-norleucine (DON) | Sigma | Cat# D2141 |
| 2-deoxy-glucose | Sigma | Cat# D3179 |
| UK5099 | Sigma | Cat# PZ0160 |
| CXCL-10 | Peprotech | Cat# 300-12 |
| CCL19 | Peprotech | Cat# 300-29B |
| CCL20 | Peprotech | Cat# 300-29A |
| IL-7 | Peprotech | Cat# 200-07 |
| IL-15 | Peprotech | Cat# 200-15 |
| Critical Commercial Assays | ||
| ENLITEN ATP Assay System | Promega | Cat# FF2000 |
| Experimental Models: Cell Lines | ||
| 293T | ATCC | CRL-3216 |
| Oligonucleotides | ||
| LRT F: TGT GTG CCC GTC TGT TGT GTG A | Taylor et al., 2015 | N/A |
| LRT R: GAG TCC TGC GTC GAG AGA TCT | Taylor et al., 2015 | N/A |
| 2LTR F: AAC TAG GGA ACC CAC TGC TTA AG | Taylor et al., 2015 | N/A |
| 2LTR R: TCC ACA GAT CAA GGA TCT CTT GTC | Taylor et al., 2015 | N/A |
| GAPDH F: GAA GGT GAA GGT CGG AGT | Taylor et al., 2015 | N/A |
| GAPDH R: GAA GAT GGT GAT GGG ATT TC | Taylor et al., 2015 | N/A |
| Recombinant DNA | ||
| HIV-1 NL4-3 ΔEnv EGFP reporter vector | Zhou et al., 2005 | N/A |
| X4 HIV-1 envelope expression vector | Zhou et al., 2005 | N/A |
| pCMV-VSVg | Dharan et al., 2016 | N/A |
| R7ΔEnvGFP | Dharan et al., 2016 | N/A |
| Software and Algorithms | ||
| GraphPad Prism (GraphPad) | Motulsky and Brown, 2006 | https://www.graphpad.com |
| ImageJ | Schneider et al., 2012 | https://imagej.nih.gov/ij/ |
| FACSDiva software version 8.0.1 (BD Biosciences) | Becton, Dickinson and Company | https://www.bdbiosciences.com/en-us/instruments/research-instruments/research-software/flow-cytometry-acquisition/facsdiva-software |
| FlowJo software version 10 | FlowJo LLC | https://www.flowjo.com/ |