Skip to main content
. 2019 Aug 25;21(8):824–829. [Article in Chinese] doi: 10.7499/j.issn.1008-8830.2019.08.016

1.

sgRNA寡核苷酸序列

sgRNA 序列
注:蓝色序列示原间隔序列临近基序(protospacer adjacent motif)。
sgRNA1 5′-AATTTCCATTGGCGGGAC<span style="color:blue">TGG-3′</span>
sgRNA2 5′-TAAGTCCAGTCCCGCCAA<span style="color:blue">TGG-3′</span>
sgRNA3 5′-GGTTTCAATTTCCATTGG<span style="color:blue">CGG-3′</span>
sgRNA4 5′-CCGCATCACTCTGCTTGA<span style="color:blue">AGG-3′</span>
sgRNA5 5′-AACACTCATACCCATACT<span style="color:blue">TGG-3′</span>