Table 2.
Primer sequences used for the Real-Time Polymerase Chain Reaction
| Gene | Primer sequence | |
|---|---|---|
| Notch 1 | Forward | F5’-GCGACAACGCCTACCTCTG |
| Reverse | R5’-AAGCCATTGATGCCGTCC | |
| Notch 2 | Forward | F5’-TCAGCCGGGATACCTATGAG |
| Reverse | R5’-CTGGCAGTGTCCTGGAATGT | |
| Notch 3 | Forward | F5’-ACCGCGTGGCTTCTTTCTA |
| Reverse | R5’-GAGCACTCGTCCACATCCTG | |
| Notch 4 | Forward | F5’-GGAGAAGGGGCTGTGGAAT |
| Reverse | R5’-GCAGGGGTCAGGAAACTGG | |
| HES 1 | Forward | F5’-GAGTGCATGAACGAGGTGAC |
| Reverse | R5’-GGTCATGGCATTGATCTGG | |
| HIF1α | Forward | F5’-ACCACCTATGACCTGCTTGG |
| Reverse | R5’-TATCCAGGCTGTGTCGACTG | |
| GAPDH | Forward | F5’-GCACCGTCAAGGCTGAGAAC |
| Reverse | R5’-TGGTGAAGACGCCAGTGGA |