Table 3. Additional adprhl1 gRNA sequences.
| gAdprhl1-e1-1: | 5’-GGCAAGAAGAGCTAAAACAACT | direction: →, PAM: TGG |
| gAdprhl1-e1-2: | 5’-GGTGAGACTAGTGATTCCGCTG | direction: ←, PAM: TGG |
| gAdprhl1-e2-1: | 5’-GGCACACCCCATTCAATGAAAA | direction: →, PAM: AGG |
| gAdprhl1-i2-1L: | 5’-GGAAAGCACTTAGTGACCAG | direction: ←, PAM: TGG |
| gAdprhl1-i2-2L: | 5’-GGGAATGTAATAAAAATTAG | direction: ←, PAM: TGG |
| gAdprhl1-e4-2S: | 5’-GGAGAAACCACTTGCTCAGTG | direction: →, PAM: GGG |
| gAdprhl1-e4-2L: | 5’-GGAAAAACCACTTGTTCAGTG | direction: →, PAM: GGG |
| gAdprhl1-e4-3S: | 5’-GGAAGAAGACTATACGGCACA | direction: →, PAM: TGG |
| gAdprhl1-e4-3L: | 5’-GGAAGAAGACTATTCGGCACA | direction: →, PAM: TGG |
| gAdprhl1-e5-1S: | 5’-GGTTTTATTTTGAAGCCAAG | direction: →, PAM: TGG |
| gAdprhl1-e5-1L: | 5’-GGTTTTATTTTGAAACCAAG | direction: →, PAM: TGG |
| gAdprhl1-e7-1S: | 5’-GCAGGAGAAGGTGGTGCCAC | direction: →, PAM: TGG |
| gAdprhl1-e7-1L: | 5’-GCAGGAGAAGGTGGTGCTAC | direction: →, PAM: TGG |
| gAdprhl1-e7-2S: | 5’-GGTGTCTTTATGGATTGCTCTA | direction: →, PAM: TGG |
| gAdprhl1-e7-2L: | 5’-GGTGTCTGTATGGATTGCTCTA | direction: →, PAM: TGG |
-e5-1 (S and L) fits the optimal gRNA consensus but gave consistently poor synthesis yields due to an adverse T-repeat sequence and could not be assessed by injection into embryos.