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. 2020 Jul 17;16(7):e1008670. doi: 10.1371/journal.ppat.1008670

Fig 5. MARL regulates miR-122 expression and activity.

Fig 5

(A) Overexpression of MARL reduces the expression of miR-122. Transfection of pcDNA3.1 vector or MARL expression plasmid into MIC cells for 48 h. The expression of MARL and miR-122 were measured by qPCR, respectively. (B) Knockdown of MARL upregulated miR-122 expression. MIC cells were transfected with MARL-specific siRNAs (si-MARL) or si-Ctrl for 48 h. The expression of MARL and miR-122 were measured by qPCR, respectively. (C) miR-122 sensor construct. The miR-122 sensor was constructed by inserting two copies of perfectly matched miR-122 fragments into psiCHECK-2 vector. (D) MARL overexpression induced the luciferase activity. MIC cells were transfected with pcDNA3.1 vector or MARL expression plasmid, together with miR-122 sensor. At 48 h post-transfection, the luciferase activity was analyzed. (E) MARL reduces miR-122 activity. MIC cells were transfected with miR-122, pcDNA3.1 vector, or MARL expression plasmid, together with miR-122 sensor for 48 h. The luciferase activity was analyzed and normalized to renilla luciferase activity. All data represented the mean ± SE from three independent triplicated experiments. *, p < 0.05.