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. 2020 Jun 3;1(1):100010. doi: 10.1016/j.xpro.2019.100010

Figure 1.

Figure 1

Four hMGT Plasmid Variations to Choose from Depending on Experimental Needs

(A) A retrovirus polycistronic hMGT construct.

(B) A polycistronic hMGT lentiviral construct based off of the pll3.7 backbone (gifted from the lab of Qing Zhang), where hMGT is driven by a UBC promoter and puromycin is driven by an Ef1α promoter.

(C) A polycistronic hMGT lentiviral construct based off of the pll3.7 backbone, in which hMGT is driven by CAG and puromycin selection is driven by an EF1α promoter.

(D) A polycistronic hMGT lentiviral construct based off of the pLenti-GFP backbone (Addgene, cat. no. 17448), in which hMGT is driven by an Ef1α promoter and puromycin selection is driven from a PGK promoter.