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. 2020 Jun 3;1(1):100010. doi: 10.1016/j.xpro.2019.100010

Figure 2.

Figure 2

Preparation of Retroviral Particles

(A) An example of 80%–90% confluency of 293T cells. Cells should appear similarly immediately prior to transduction. Scale bar for both images is 100 μm.

(B) Viral precipitation gathering at the bottom of 50 mL tube after a 17 - 18 h incubation with PEG/PBS.

(C) After centrifugation, the viral pellet should appear as a white or beige pellet at the bottom of the 50 mL tube. All traces of liquid should be removed after second spin. The beige / white pellet should appear as shown here, without any excess liquid.