Figure 3.

Brightfield Image of Reprogrammed hiCMs

(A) Standard human cardiac fibroblasts at reprogramming day zero.

(B) 11 days after transduction, hMGT133 infected cells start to undergo some morphology changes.

(C) 14 days after reprogramming, cells that were not infected with hMGT133 appear like standard fibroblasts.

(D and E) At reprogramming day 14, fibroblasts that were infected with hMGT133 are morphologically distinct from the fibroblast appearance at day zero and have a cardiomyocyte-like appearance. Note, that depending on the density of cells in culture, the individual cell shape of hiCMs may be clearly seen in a less dense culture (D), or less clearly seen in a denser culture (E). hiCM appearance as seen in either (D) or (E) is fine and is due the final density of cells. Scale bar for all images is 100 μm.