REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse monoclonal to cardiac troponin T	ICC: abcam clone 1F11 Flow: Thermo Fisher clone: 3-11	ICC: Cat# ab10214; RRID:AB_2206574 Flow: Cat# MS-295-P; RRID:AB_61806
Mouse monoclonal to a-Actinin (clone EA-53)	sigma	Cat# A7811; RRID:AB_476766
Donkey anti-mouse IgG(H+L), AlexaFluor647 conjugated	Jackson Lab	Cat# 715-605-151; RRID:AB_2340863
Donkey anti-rabbit IgG(H+L), AlexaFluor488 conjugated	Jackson Lab	Cat# 711-545-152; RRID:AB_2313584
Bacterial and Virus Strains
E.coli: HB101	Promega	L2015
E.coli: XL10-Gold Ultracompetent cells	Agilent	200315
Biological Samples
Human cardiac tissue from a male patient	Duke Human Heart Repository	https://sites.duke.edu/dhhr/
Chemicals, Peptides, and Recombinant Proteins
Phosphate-buffered saline (PBS)	Fisher BioReagents	BP399-20
Poly-l-lysine	Electron microscopy science (EMS)	19321-B
DMEM	Corning	10-013-CV
IMDM	GIBCO	21980
DMEM/F12	GIBCO	11320-033
mTeSR1 medium	STEMCELL Technologies	85850
FBS	Millipore	TMS-013-B
Non-essential amino acids	Corning	25-025-CI
Penicillin/streptomycin	Corning	30-002-CI
PEG6000	Sigma	81255-2.5g
0.05% Trypsin	Corning	25-052-CI
M199	Corning	10-060-CV
Polybrene	Millipore	TR-1003-G
SurCoat	Celltron	SC-9035
RPMI1640	gibco	11875-093
B27 supplement	gibco	17504-044
BSA	Hyclone	SH3057402
GlutaMAX	gibco	35050-061
Gelatin	sigma	G1393-100ml
Dispase	STEMCELL Technologies	07923
Ascorbic acid	Sigma-Aldrich	A-4544
Puromycin	Thermo Fisher	A1113803
Critical Commercial Assays
NanoFect transfection Reagent	Alstem	NF101
Experimental Models: Cell Lines
Human: 293LTV Cell Line	Cell Biolabs	LTV-100; RRID: CVCL_JZ09
Human: ESC H9 derived fibroblasts: H9F	(Fu et al., 2013)	N/A
Human: primary cardiac fibroblasts: HCF1	(Zhou et al., 2019)	N/A
Recombinant DNA
pMXs-puro-hMGT	(Zhou et al., 2019)	N/A
pBabe-miR-133	(Nam et al., 2013)	N/A
pLL3.7-UBCp-Ef1a-pur-Kan	Gift - Lab of Qing Zhang	N/A
pLL3.7-UBCp-hMGT-Ef1a-puro	This paper	N/A
pLL3.7-CAG-hMGT-Ef1a-puro	This paper	N/A
pLenti-CMV-GFP-Puro (658-5)	Addgene	17448; RRID:Addgene_17448
pLenti-Ef1a-hMGT-PGK-puro	This paper	N/A
pMXs-puro-dsRed	(Wang et al., 2015a)	N/A
pMXs-GFP	(Wang et al., 2015a)	N/A
pMXs-tdTomato	(Liu et al., 2017a), (Liu et al., 2017b)	N/A
pCMV-VSV-G	Addgene	8454; RRID:Addgene_8454
Gag-Pol Retroviral Vector	Cell Biolabs, Inc.	RV-11
psPAX2	Addgene	12260; RRID:Addgene_12260
pMD2.G	Addgene	12259; RRID:Addgene_12259
Software and Algorithms
EVOS microscope system	Invitrogen	N/A
Other
Retro-X qRT-PCR titration kit	Clontech	631453