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. 2020 Jun 3;1(1):100010. doi: 10.1016/j.xpro.2019.100010
Retrovirus Lentivirus
(1) DNA / Plasmid Mix (1) DNA / Plasmid Mix
5 μg gag/pol(Ieda et al., 2010) 7 μg psPAX2
2.5 μg VSVG 3 μg pMD2.G
15 μg hMGT OR miR-133 10 μg hMGT
In 500 μl DMEM In 500 μl DMEM
(2) NanoFect Mix (2) NanoFect Mix
45 μl NanoFect 45 μl NanoFect
In 500 μl DMEM In 500 μl DMEM