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. Author manuscript; available in PMC: 2020 Nov 1.
Published in final edited form as: ACS Catal. 2019 Sep 17;9(11):9775–9788. doi: 10.1021/acscatal.9b02737

Figure 4. Directed evolution of enzymes using structure-based recombination.

Figure 4.

(a) Generation of chimeric enzymes via SCHEMA-guided recombination of fragments derived from homologous parent sequences and chosen to minimize structural disruption.17 (b) SCHEMA-guided recombination of β-lactamase PSE-2 and TEM-1 produces a higher fraction of functional chimeras compared to random mutagenesis.62 (c) Chimeric P450s obtained via SCHEMA-guided recombination of P450 BM3 (CYP102A1) with its homologous enzymes CYP102A2 and CYP102A3 can exhibit higher stability against thermal denaturation compared to any of the parent P450s.65 Adapted with permission from ref. 62. Copyright 2005, National Academy of Sciences.