Figure 7.
Artemisinin restored the mitochondrial membrane potential, and decreased ROS accumulation and reduced apoptosis induced by Aβ1-42 in SH-SY5Y cells. (A) Cells were pretreated with 12.5μM ART for 120min and then induced with or without 4μM Aβ1-42 for a further 24h. The decline in the mitochondrial membrane potential was reflected by the shift of fluorescence from red to green indicated by JC-1. Intracellular ROS level was measured by the CellROXs Deep Red Reagent. Nuclear morphology measured by Hoechst staining (40x). (B-D) Statistical analysis results of JC-1, ROS and Hoechst staining in A. (E) Photographs of representative cultures measured by Flow cytometry. (F) Statistical analysis results of Flow cytometry in E. (G) Expression of Bax, Bcl-2, and GAPDH were detected with Western blotting. (H) Quantification of representative protein band from Western blotting in G. *P<0.05 or **P<0.01, or ***P<0.001, CTL vs Aβ1-42; #P<0.05 or ##P<0.01 or ###P<0.001 Aβ1-42 vs Aβ1-42+ART.