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Stopped-flow (SF) absorption kinetic traces at 320 nm of Mn/Fe cofactor assembly in (A) Y175F, (B) WT, and (C) E69D R2lox as a function of metal concentration. FeII was held at 0.75 equiv of apo-R2lox and formation of the resting state monitored at 320 nm with increasing [MnII]. (Insets) Corresponding SF kinetic traces for FeII competition experiments.
