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. Author manuscript; available in PMC: 2021 Mar 18.
Published in final edited form as: J Am Chem Soc. 2020 Mar 9;142(11):5338–5354. doi: 10.1021/jacs.0c00333

Figure 9.

Figure 9.

(A) Cyclic voltammograms of WT Mn/Fe R2lox from pH 6 to pH 9 (as indicated). (Inset) Anodic (open symbols) and cathodic (closed symbols) peak potentials for WT Mn/Fe R2lox as a function of pH. Dotted lines indicate a slope of −120 mV/pH unit. (B) Cyclic voltammograms of Mn/Fe (solid) and apo (dashed) R2lox at pH 7.0. (C) Anodic (open symbols) and cathodic (closed symbols) peak potentials as a function of scan rate. All samples contained 100 μM WT (black), Y175F (blue), or E69D (green) R2lox protein with 100 mM NaCl in 100 mM HEPES buffer, pH 8.0. Points and error bars reflect the average and standard deviation of n ≥ 3 trials, respectively. Samples measured with a glassy carbon working electrode.