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. 2020 Jul 24;133(14):jcs239814. doi: 10.1242/jcs.239814

Fig. 6.

Fig. 6.

Rab1 regulates release of cargo carriers from ERES. (A) Representative examples of cargo localization before or 20 min after biotin treatment in COS-7 cells expressing mCh-TNF RUSH (red), BFP-Rab1a or BFP-Rab1a N124I (green) and GFP-Sec61β (gray). (B) As in A, but for BFP-Rab1b or BFP-Rab1b N121I. (C,D) Quantification of TNF cargo recruitment to the Golgi was measured by tracking fluorescence intensity measured within a 10×10 μm2 ROI around the Golgi region (perinuclear area, marked by anti-Giantin) versus peripheral ER at 0 and 20 min following biotin addition in the presence of either BFP-Rab1a, BFP-Rab1a N124I, BFP-Rab1b or BFP-Rab1b-N121I. Redistribution of cargo to the perinuclear/Golgi region was quantified by plotting the ratio of fluorescence intensity of TNF cargo between the perinuclear and peripheral ROIs (***P<0.001). Error bars represent s.e.m. Scale bars: 5 µm.