Table 1.
Oligos used for qPCR, morpholinos, and ISH probe templates
| Primer | Application | Sequence (5′–3′) |
|---|---|---|
| Ax_vwde_ISH_F | Riboprobe template | TGTGGAAAGAAACTTGTGCATCA |
| Ax_vwde_ISH_R | Riboprobe template | TTTAATCTGAAAATGGACCAGTAGATT |
| vwde MO1 | Antisense morpholino | ATATCCCATACATCCTTGCGTTGGC |
| vwde MO2 | Antisense morpholino | AGAAACCATCACAGTTCCTCACAGT |
| vwde MO1 inverted (control (INV)) | Control morpholino | CGGTTGCGTTCCTACATACCCTATA |
| Standard control MO (control (SC)) | Control morpholino | CCTCTTACCTCAGTTACAATTTATA |
| Ps_Vwde‐qPCR_F | qPCR | AGAATTCCTGTGACTGTGCGA |
| Ps_Vwde‐qPCR_R | qPCR | TTCTGGTGTTGTTGGTGAGGG |
| Ps_Vwde‐ISH_F | Riboprobe template | GGCCGCGGGCATGCGGAATAATGTGTGCT |
| Ps_Vwde‐ISH_R | Riboprobe template | CCCGGGGCAGTCCAGTCTTCAGCAGTGTG |
| Lp_Vwde‐qPCR_F | qPCR | TTCTTCTTGGAGACCCCTGAT |
| Lp_Vwde‐qPCR_R | qPCR | GGTCTTGCTGGCTAGTGTCAG |
| Lp_Vwde‐ISH_F | Riboprobe template | GGCCGCGGAGCTAACAGCCTGTGCAACAT |
| Lp_Vwde‐ISH_R | Riboprobe template | CCCGGGGCATCAGGGGTCTCCAAGAAGAA |
| 3′_T7 universal | Riboprobe template, 2nd‐round PCR | AGGGATCCTAATACGACTCACTATAGGGCCCGGGGC |
| 5′_T7 universal | Riboprobe template, 2nd‐round PCR | GAGAATTCTAATACGACTCACTATAGGGCCGCGG |
Abbreviations: ISH, in situ hybridization; qPCR, quantitative polymerase chain reaction.