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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: Neuroscience. 2020 Jun 13;441:33–45. doi: 10.1016/j.neuroscience.2020.06.009

Figure 1. VPS35 D620N inhibits autophagy.

Figure 1.

(A) Transgenic and endogenous VPS35 were evaluated by western blot. RA-differentiated SH-SY5Y cells stably expressing transgenic VPS35 (WT or D620N) were treated with or without bafilomycin A1. (B) Western blot of LC3-II was performed and (C) autophagic flux was quantified. Total protein loading was confirmed with β-Actin expression obtained from the same membrane. Results from three independent experiments are displayed (*p<0.05 compared to indicated group, student’s t-test; error bars = SEM).