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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: Neuroscience. 2020 Jun 13;441:33–45. doi: 10.1016/j.neuroscience.2020.06.009

Figure 2. VPS35 knockdown inhibits autophagy.

Figure 2.

(A) VPS35 expression was knocked down in differentiated SH-SY5Y cells neurons by two distinct lentivirus-delivered shRNA vectors. Following selection with puromycin, transduced cells were treated in the presence or absence of bafilomycin A1. Western blot of LC3-II was performed and (B) autophagic flux quantified. Results from three independent experiments are shown (*p<0.05 versus indicated group, student’s t-test; error bars = SEM). (C) Knockdown of VPS29 by shRNA using a lentiviral vector was also performed. Representative image groupings were obtained from single western blot membranes probed with multiple antibodies as indicated. Total loading for all western blot membranes was assessed using β-Actin.