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. Author manuscript; available in PMC: 2021 Aug 10.
Published in final edited form as: Neuroscience. 2020 Jun 13;441:33–45. doi: 10.1016/j.neuroscience.2020.06.009

Figure 4. HA inhibits autophagy.

Figure 4.

(A) RA-differentiated SH-SY5Y cells were treated with high molecular weight (>950 kDa) HA in serum-free media for 24 hours. The next day, cells were treated with or without bafilomycin A1 and autophagic flux was assessed by LC3-II/β-Actin western blot and (B) quantified from three experiments. (C) Similarly, low molecular weight (15 – 40 kDa) HA treatment was performed and autophagic response was evaluated by LC3-II/β-Actin western blot and (D) represented graphically (*p<0.05 versus indicated group, student’s t-test; error bars = SEM).